Description
Intended Uses: For the quantitative detection of Rabbit Sclerostin (SOST) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SOST in Rabbit serum, plasma, tissue homogenates. An antibody specific for SOST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOST present is bound by the immobilized antibody. After removing any unbound substances, a biotinconjugated antibody specific for SOST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOST bound in the initial step. The color development is stopped and the intensity of the color is measured